Xenbase Image ID: 147164

Figure 3: Repeating pattern of non-RVD variations enhances the activity of TALENs. (A) Schematic design of TALENs used in this assay. Red bars indicate left and right TALENs. Red lines and letters indicate spacer regions. The target sequences of ATM and APC were originally described by Reyon et al. The target sequence of eGFP was originally described by Sakuma et al (B) SSA assay for four types of TALEN targeting three genes. Data are expressed as means ± SEM (n = 2). (C) Cel-I assay for four types of TALEN targeting ATM and APC. Arrowheads indicate the expected positions of the digested products. NHEJ (nonhomologous end joining) was estimated using ImageJ software as previously described. Image published in: Sakuma T et al. (2013) Copyright © 2013, Macmillan Publishers Limited. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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