Xenbase Image ID: 147165

Figure 4: Highly efficient targeted gene disruption in Xenopus laevis using Platinum TALENs. (A) Schematic design of TALEN used in this assay. Red bars indicate left and right TALENs. Red lines and letters indicate spacer region. (B) Phenotypes of uninjected and Platinum tyr TALEN-injected embryos. Embryos were reared to the hatching stage. An asterisk indicates sequenced embryo related to Supplementary Fig. S3. (C) Percentages of phenotypes in the uninjected and Platinum tyr TALEN groups. Total numbers of individuals in this experiment are shown at the top of each graph. Strong, near complete loss of pigmentation in retina pigmented epithelium (RPE); Moderate, more than half loss of pigmentation in RPE; Weak, less than half loss of pigmentation in RPE; Normal, no alteration of pigmentation. Representative phenotypes were shown in a previous report12. (D) RFLP analysis of tyr paralogs. Due to its allotetraploid genome, there are two paralogs, tyr a and tyr b, in X. laevis. PCR products of tyr a (left panel) and tyr b (right panel) paralogs were purified, digested by HinfI and analyzed by agarose gel electrophoresis. Image published in: Sakuma T et al. (2013) Copyright © 2013, Macmillan Publishers Limited. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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