XB-IMG-147171
Xenbase Image ID: 147171
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Figure 1. PTK7 interacts with ROR2.
(A) PTK7, FLAG-ROR1 and FLAG-ROR2 were co-expressed in HEK 293T cells. Proteins extracted
from cell lysates were immunoprecipitated with anti-PTK7 antibody and revealed with the mentioned
antibodies. (B) Schematic diagram of PTK7 constructs used in this study. KN: kinase domain. (C) and
(D) FLAG-ROR2 was co-expressed in HEK 293T cells with the constructs expressing the truncated
forms of PTK7 and co-immunoprecipitation were done as in (A). (E) Cytosol/membrane fractionation
of HEK 293T cells expressing the truncated forms of PTK7. Cell lysates were obtained using
hypotonic lysis buffer. Samples were centrifuged to obtain cytosolic fractions. Membrane fractions
were obtained by ultracentrifugation (1h, 40 000 rpm) of the cytosolic fraction. -tubulin was used as
cytosolic control. (F) Proteins extracted from MEFs stimulated or not with WNT5A (200 ng/ml) were
subjected to co-immunoprecipitation with anti-PTK7 (PTK7) antibody or an isotype matched control
antibody (IgG). After western blot, total cell lysates (TCL) and immunoprecipitated proteins were
probed with the mentioned antibodies. Image published in: Martinez S et al. (2015) © 2015 by The American Society for Biochemistry and Molecular Biology, Inc. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |