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Figure 2. PTK7 interacts with WNT5A. (A) Flag-ROR2 and MYC-WNT5A were co-expressed in HEK 293T cells. Cell lysates were subjected to co-immunoprecipitation with anti-FLAG antibody and then submitted to western blot with the mentioned antibodies. FLAG-NECTIN4 was used as a negative control. (B) FLAG-PTK7 was coexpressed with HA-WNT1 or HA-WNT5A in HEK 293T cells. Co-immunoprecipitations were done as in (A) (C) Full and truncated isoforms of PTK7 were co-expressed with MYC-tagged WNT5A in HEK 293T cell. Cell lysates were subjected to co-immunoprecipitation with anti-MYC antibody and then submitted to western blot with the mentioned antibodies. (D) PTK7-FLAG and and HA-WNT5A were co-expressed in HEK 293T cells transfected or not with a siRNA directed against ROR2. Cell lysates were subjected to co-immunoprecipitation with anti-FLAG antibody followed by western blot analysis. (E) PTK7, FLAG-ROR2 and HA-WNT5A were co-expressed in HEK 293T cells. Cell lysates were subjected to co-immunoprecipitation with anti-HA antibody followed by western blot analysis. FLAG-CDC37 was used as a negative control. Image published in: Martinez S et al. (2015) © 2015 by The American Society for Biochemistry and Molecular Biology, Inc. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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