Xenbase Image ID: 147174

Figure 4. Ptk7 cooperates with Ror2 during Xenopus embryo morphogenesis. (A) Whole-mount in situ hybridization (WISH) of ptk7 in early (st.10), mid (st.11) and late (st.12) gastrula embryos. (B) Two-cell embryos were injected in one single blastomere with 10 ng of Ptk7- MO and processed for WISH of papc at mid-gastrula stage (C) Two-cell embryos were injected in each blastomere with 10 ng of Ptk7-MO or 40 ng of Ror2-MO. Morphology was analyzed at tailbud stage. (D) Two-cell embryos were injected in each blastomere with the indicated amounts of Ptk7-MO and Ror2-MO. Morphology was analyzed at tailbud stage. The number of injected embryos is indicated above the bars. Class I embryos are morphologically normal, class II embryos are shorter and have a wider neural plate, class III embryos show severe neural tube closure defects. (E) Embryos injected as in (D) were collected at late gastrula (st.13) and processed for RT-qPCR. For all qPCR graphs, error bars represent s.e.m. values of three independent experiments with two technical duplicates. For statistical analyses, samples were compared with the respective Ctrl using unpaired Student’s t-test. Image published in: Martinez S et al. (2015) © 2015 by The American Society for Biochemistry and Molecular Biology, Inc. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue pcdh8.L papc, paraxial protocadherin, xpapc X. laevis Throughout NF stage 11 dorso-lateral marginal zone ptk7.L cck-4, cck4, klg, ptk-7 X. laevis Sometime during NF stage 10 to NF stage 12 animal hemisphere Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + ptk7 MO + NF11 (in situ hybridization) fig.4.b Expression Phenotype decreased amount pcdh8.L expression in upper blastopore lip Xla Wt + ptk7 MO + tailbud stage (morphology) fig.4.c Anatomical Phenotype abnormal incomplete closing of the neural tube decreased length of anterior-posterior axis increased width of neural plate Xla Wt + ror2 MO + tailbud stage (morphology) fig.4.c Anatomical Phenotype abnormal incomplete closing of the neural tube wholly ventralized embryo Xla Wt + ptk7 MO + NF13 (RT-PCR) fig.4.e Expression Phenotype decreased amount pcdh8.L expression in whole organism Xla Wt + ror2 MO + NF13 (RT-PCR) fig.4.e Expression Phenotype decreased amount pcdh8.L expression in whole organism Larger Image Printer Friendly View

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