XB-IMG-147175
Xenbase Image ID: 147175
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Figure 5. Rescue assays of Ptk7 morphant phenotypes by human PTK7 constructs.
(A) and (B) Two-cell embryos were injected in each blastomere with 10ng of Ptk7 MO followed by
injection at the four-cell stage of synthetic transcripts (200pg total for each RNA based on initial dose response
tests) encoding the various human PTK7 constructs used in this study (see Fig. 1B). (A)
Blastopore closure was estimated using the ratio of blastopore diameter to the mean of control (Ctrl)
blastopore diameter at st.13. Bars represent maximum and minimum values, and the line represents the
mean. 15 to 27 embryos per condition were used for the analysis. (B) Representative blastopore
closure phenotypes recorded (vegetal views, the blastopore is delineated in red). Note that only fulllength
human PTK7 and 1-7 PTK7 could significantly rescue blastopore closure of Ptk7 morphant
embryos. (C) Embryos injected as in (A) were processed for RT-qPCR at stage 13 for papc
expression. Full-length human PTK7 and 1-7 PTK7 could significantly reactivate papc expression in
morphant embryos. For statistical analysis of blastopore closure, samples were compared with the
respective Ctrl (* over the bar) and Ptk7-MO (* over the line) using unpaired Student’s t-test. For the
qPCR graph, error bars represent s.e.m. values of two independent experiments with two technical
duplicates. For RT-qPCR statistical analysis, samples were compared with the respective Ctrl (* over
the bar) and Ptk7-MO (* over the line) using unpaired Student’s t-test (*P< 0.05; **P< 0.005; ***P<0.005). Image published in: Martinez S et al. (2015) © 2015 by The American Society for Biochemistry and Molecular Biology, Inc. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
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