Xenbase Image ID: 147219

Figure 5. Highly efficient targeted gene disruption in rats using Platinum TALENs.(A) Schematic design of TALEN used in this assay. Red bars indicate left and right TALENs. Red lines and letters indicate spacer region. (B) Cel-I assay for ZFN- or TALEN-induced mutations in rat Il2rg gene. Arrowheads indicate the expected positions of the TALEN-digested products. % NHEJ (nonhomologous end joining) was estimated using ImageJ software as previously described29. Data are expressed as means ± SEM (n = 3). *P < 0.01 by Student's t-test: Il2rg ZFN vs. Platinum Il2rg TALEN. (C) Microinjection of ZFNs or TALENs targeting Il2rg into fertilized eggs of F344 rats. *P < 0.01 by chi-square test: Il2rg ZFN vs. Platinum Il2rg TALEN. (D) Sequence analyses of TALEN-induced mutant rats. Blue letters indicate TALEN target sites. Gaps generated by deletion are shown as dashes in red. Image published in: Sakuma T et al. (2013) Copyright © 2013, Macmillan Publishers Limited. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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