Xenbase Image ID: 147363

Fig 2. Down-regulation of VICKZ enhances CNC delamination and causes NT dissociation.2-4ss embryos were electroporated with either control GFP (A,G), Y396F-GFP (B,F,H), FITC labeled control morpholino (CMO) (C) or FITC labeled cVICKZ-antisense morpholino (AMO) (D) and fixed and sectioned either 10 hours (A,B,C,D,F) or 20 hours (G,H) post-electroporation. All sections were stained for HNK-1 expression (red) and GFP (green). (E) For each treatment, the percentage of the total number of GFP-positive cells that delaminated post-electroporation was calculated for each embryo (see Materials and Methods) and graphed as the mean ± SEM (N = 5). Y396F-treated embryos showed a 2.4-fold increase in delamination as compared to the controls; AMO-treated embryos showed a 2.8-fold increase in delamination compared to CMO-treated embryos. Insets in D, F and H (inset 2) show enlarged images of the nuclei in the corresponding rectangles indicated by the dotted lines. Inset 1 in H shows the red channel (HNK-1) outlined by the rectangle. Arrowheads indicate emigrating, electroporated CNC (B,C,D). Arrows in (D) indicate disorganization of the electroporated neuroepithelium. Image published in: Carmel MS et al. (2015) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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