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Fig 3. Overexpression of cVICKZ1 inhibits CNC emigration.2-4ss embryos were electroporated with either control GFP (A,C) or cVICKZ1-GFP (B,D), and fixed 20 hours post-electroporation. Embryos were stained for HNK-1 expression (red) and GFP (green) and photographed either as whole mount preparations (A,B) or after sectioning (C,D). The grey dotted line in (A) and (B) indicate the plane of section shown in (C) and (D), respectively. Almost no CNC delamination occurs in the cVICKZ1-GFP embryos, although GFP-negative, HNK-1-positive cells emigrate normally. (E) The percentage of the total number of GFP-positive cells that delaminated by 20 hours post-electroporation was calculated for each embryo and was graphed as the mean ± SEM (N = 5). Abbreviations: E, eye. Image published in: Carmel MS et al. (2015) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |