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Fig 4. Down-regulation of VICKZ causes DM dissociation.(A-C) VICKZ is expressed in newly-formed somites in E2 embryos (A) particularly in the apical adherens junctions, and later in the early dermomyotome (B). In E3 embryos, VICKZ is down-regulated in the emerging dermis but maintained in the developing myofiber (C). (VICKZ is also down-regulated in the emigrating trunk NC cells (arrows in B)). (D-G) Embryos were electroporated with either control GFP (D, F) or Y396F-GFP (E, G) into the dorsal medial lip (DML) of early dissociating somites. Embryos were fixed either 12 (D,E) or 20 (F,G) hours post-electroporation and stained for the DM marker, Pax 7 (purple) and/or the myotome marker, Desmin (red). After 12 hours, control GFP-labeled DML cells retain their epithelial structure and are Pax7-positive (D) whereas many DML cells that received Y396F-GFP have begun rounding up and losing their epithelial pseudostratified morphology (E, arrowheads). By 20 hours post-electroporation, in the control-GFP embryos, GFP-positive, Desmin-positive myofibers derived from the DML occupy the medial-most aspect of the myotome (F, arrows). In Y396F-GFP treated embryos, however, the DM was completely dissociated, with GFP-positive, Pax7-negative cells having lost their epithelial morphology. None of the GFP-positive cells generated myofibers. Abbreviations: ES, epithelial somite; DM, dermomyotome; D, dermis; M, myotome. Image published in: Carmel MS et al. (2015) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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