Xenbase Image ID: 147433
FIGURE 2:. Mgc MO, MO + MgcS386E, and MO + MgcR384A exhibit cytokinesis failure and significant delays in cytokinesis completion in vivo. (A) Frames from time-lapse movies during cytokinesis. Embryos were coinjected with the indicated MO and rescue constructs along with mChe-UtrCH (F-actin probe) and mChe-H2B. Red arrowheads point to the contractile ring during early cytokinesis; red arrows indicate cleavage furrow regression; yellow arrowheads point to F-actin–rich junctional accumulations. (B) Quantification of the outcome of cytokinesis showing the percentage of cells that complete cytokinesis normally, fail cytokinesis with cleavage furrow regression, or fail cytokinesis with formation of multiple cleavage furrows. Control (number of cells, embryos, and independent experiments, respectively [n] = 26, 9, 4), MO (n = 29, 8, 4), MO + WT (n = 20, 7, 4), MO + S386A (n = 53, 13, 5), MO + S386E (n = 48, 12, 5), and MO + R384A (n = 19, 3, 3). (C) Quantification of cytokinesis duration. Error bars represent SEM. The p values were calculated from pairwise comparison to MO + MgcWT. Control (n = 20, 7, 5), MO (n = 17, 8, 7), MO + WT (n = 17, 11, 7), MO + S386A (n = 22, 12, 5), MO+S386E (n = 20, 9, 4), and MO + R384A (n = 20, 9, 4). (D) Frames from time-lapse movies of cells expressing mChe-UtrCH (F-actin probe) that do manage to complete cytokinesis successfully show that the MO, MO + MgcS386E, and MO + MgcR384A cells display significantly longer cytokinesis duration than control or MO + MgcWT. Scale bars, 20 μm.
Image published in: Breznau EB et al. (2015)
Image downloaded from an Open Access article in PubMed Central. © 2015 Breznau et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).
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