XB-IMG-147436
Xenbase Image ID: 147436
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FIGURE 5:. Disrupting MgcRacGAP's GAP activity results in dynamic junctional accumulations of F-actin and RhoA-GTP. (A) Quantification of the average percentage of cells with F-actin junctional accumulations per minute. Error bars represent SEM. Control (number of cells, embryos, and independent experiments, respectively [n] =335, 10, 8), MO (n = 128, 10, 6), MO + WT (n = 202, 10, 8), and MO + R384A (n = 163, 10, 4). (B) Brightest point projection images were used to generate kymographs across a junction where an F-actin accumulation occurs in MO + R384A embryos. Kymographs of RhoA-GTP (green) and F-actin (red). The white rectangle indicates the region of the kymograph. Scale bar in en face view, 10 μm. (C) Quantification of the normalized F-actin junctional accumulation intensity over time (n = 8, 4, 4). (D) Quantification of the normalized RhoA-GTP junctional accumulation intensity over time for the same regions quantified in C. (E) Brightest point projection images were used to generate kymographs across a junction where an F-actin accumulation occurs in MO + R384A embryos. Kymographs of Rac1-GTP (green) and F-actin (red). The white rectangle indicates the region of the kymograph. Scale bar in en face view, 10 μm. (F) Quantification of the normalized F-actin junctional accumulation intensity over time (n = 8, 5, 3). (G) Quantification of the normalized Rac1-GTP junctional accumulation intensity over time for the same regions quantified in F. Notice that there is not an increase in local Rac1-GTP intensity at the site of F-actin junctional accumulations. Image published in: Breznau EB et al. (2015) © 2015 Breznau et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |