Xenbase Image ID: 147438

FIGURE 7:. MgcRacGAP is not required for proper TJ structure. (A) Fixed en face or side view images of mosaic embryos, where all cells in the field of view are stained for endogenous claudin (red), and the cells expressing GFP-membrane marker (green) contain marker alone (control) or marker + MO (MO). White lines represent separation between mosaic regions. Red arrowheads indicate claudin localization in control regions of the embryo, and green arrowheads indicate claudin localization in regions expressing the injection marker. Scale bars, 20 μm. (B) Quantification of normalized claudin intensity along the apical-to-basal axis of bicellular junctions. Error bars represent SEM. Control (number of cells, embryos, and independent experiments, respectively [n] =16, 5, 3), MO (n = 14, 5, 3). (C) Fixed en face or side view images of mosaic embryos, where all cells in the field of view are stained for endogenous ZO-1 (green), and the cells expressing mChe-membrane marker (red) contain marker alone (control) or marker + MO (MO). White lines represent separation between mosaic regions. Green arrowheads indicate ZO-1 localization in control regions of the embryo, and red arrowheads indicate ZO-1 localization in regions expressing the injection marker. Scale bars, 20 μm. (D) Quantification of normalized ZO-1 intensity along the apical-to-basal axis of bicellular junctions. Error bars represent SEM. Control (n = 15, 6, 3), MO (n = 13, 5, 3). Image published in: Breznau EB et al. (2015) © 2015 Breznau et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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