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Figure 2—figure supplement 1. Validation of Yap-MO efficiency and specificity. (A) Western blot analysis showing YAP expression decrease at different stages following microinjection of either Yap-5-mismatch-MO (control) or Yap-MO. α-tubulin is used as a loading control. (B) Lateral views and dissected eyes of stage 40 tadpoles following two-cell stage microinjection of Yap-5-mismatch-MO + ß-gal mRNA (control), Yap-MO + ß-gal mRNA (Yap-MO), Yap-5-mismatch-MO + Yap mRNA (Yap), Yap-MO + Yap mRNA (Yap-MO + Yap). (C) Quantification of dissected eye area. The Yap-MO-induced small eye phenotype is rescued by co-injection of Yap mRNA. Of note a suboptimal dose of Yap mRNA was used for the rescue experiment so that it does not alone give any eye phenotype. The number of analyzed tadpoles is indicated for each bar. Scale bar = 1 mm. DOI: http://dx.doi.org/10.7554/eLife.08488.008 Image published in: Cabochette P et al. (2015) © 2015, Cabochette et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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