Xenbase Image ID: 147468

Figure 6. Effects of Yap knockdown in the neural tube.(A) Immunostaining with anti-YAP antibody on stage 42 sections. The left side of the neural tube is delineated with yellow dotted line. A higher magnification of the ventricular zone (white dotted line) is provided in the right panel. YAP labeling is most strongly detected in this region where progenitor cells reside (arrows). (B) Analysis of EdU-labeled replication foci (1 hr-pulse) in the neural tube of stage 40 tadpoles following two-cell stage microinjection of Yap-5-mismatch-MO (control) or Yap-MO. Enlargements (dotted lines) are shown on the right. Early (red arrows) and mid/late profiles (white arrows) were distinguished. (C) Corresponding quantification. The number of analyzed tadpoles per condition is indicated in each bar. Data are represented as mean ± SEM. (D, E) In situ hybridization analysis of c-Myc or p53 expression in the neural tube of stage 40 tadpoles injected as in (B). Note the strong upregulation in the ventricular zone of the neural tube (black arrows). Scale bars = 40 µm.DOI: http://dx.doi.org/10.7554/eLife.08488.013 Image published in: Cabochette P et al. (2015) © 2015, Cabochette et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue yap1.S xyap, yap, yap2, yap65, yki X. laevis Throughout NF stage 40 ciliary marginal zone retinal stem cell region tp53.L p53, Trp248, tumor suppressor p53, Xp53, Xrel3 X. laevis Throughout NF stage 40   myc.S c-myc, cmyc, myc-a, myc-b, X-cmyc, Xcmyc X. laevis Throughout NF stage 40   Image source: Published Larger Image Printer Friendly View

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