XB-IMG-147471
Xenbase Image ID: 147471
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Figure 9—figure supplement 1.
Validation of pknox1-MO efficiency and specificity.
(A) Schematic representation of the chimeric construct containing GFP downstream of pknox1-MO complementary sequence (pknox1(5′)-GFP). (B) GFP fluorescence analysis at stage 18 following two-cell stage microinjection of GFP mRNA + pknox1-5-mismatch MO (control), GFP mRNA + pknox1-MO (pknox1-MO), pknox1(5′)-GFP + pknox1-5-mismatch-MO (pknox1(5′)-GFP) or pknox1(5′)-GFP + pknox1-MO. Fluorescence imaging at 594 nm detects the MO-bound lissamine tag. pknox1-MO efficiently inhibits GFP translation of pknox1(5′)-GFP mRNA. (C) Lateral views and dissected eyes of stage 40 tadpoles following two-cell stage microinjection of pknox1-5-mismatch-MO + ß-gal mRNA (control), pknox1-MO + ß-gal mRNA (pknox1-MO), pknox1-5-mismatch-MO + pknox1 mRNA (pknox1), pknox1-MO + pknox1 mRNA (pknox1-MO + pknox1). (D) Quantification of dissected eye area. The pknox1-induced small eye phenotype is rescued by co-injection with pknox1 mRNA. The number of analyzed tadpoles is indicated for each bar. Scale bar = 1 mm. Image published in: Cabochette P et al. (2015) © 2015, Cabochette et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |