Xenbase Image ID: 147632

Fig. 5. Increase in Smad1 following introduction of the target protector MO. (A) Representative experiment showing accumulation of Smad1 protein in the presence of increasing amounts of TPMO. Embryos were injected with TPMO in the amounts indicated and collected at st. 10.5. (B) Quantification of Smad1 accumulation as shown in (A). N=3 independent experiments. (C) Inhibition of organizer gene expression in embryos injected with 20 ng/embryo TPMO (TP-miR26 or TP mispair “Mis”). (D) Effects of the Smad1 TPMO (20 ng/embryo) on expression of direct (vent2, gata2) and indirect (vent1) targets of Smad1. (E) Partial network of Smad1-dependent transcription. The effects of inhibiting the interaction between the Smad1 mRNA and miR-26 are amplified at successive stages of the network. N≥3 biological replicates for Q-RT-PCR experiments. ⁎ Indicates statistical significance (p<0.05); ⁎⁎, very significant (p<0.01). Image published in: Liu C et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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