Xenbase Image ID: 147633

Fig. 6. Phenotypic effects of blocking the interaction between Smad1 and miR-26. (A) Uninjected embryo. (B–D) Sample phenotypes of embryos injected w/20 ng TPMO. Dorso-anterior defects are observed in approximately 25% of embryos, compared with approximately 7% of embryos injected with the 5-base mispair (165 embryos in 4 independent experiments). (E–J) in situ hybridization of not1 (E,H), otx2 (F,I), (G,J) in embryos injected with either the TPMO or the mispair (TPmis). In situ patterns shown reflect embryos from ≥3 biological replicates and are characteristic of at least 70% of embryos. For not1 expression, the pattern shown for TPMO embryos was observed in 30/37 embryos (81%); TPmis, 36/38 embryos (95%). For otx2 in gastrulae, the expression pattern shown for TPMO was observed in 19/24 embryos (79%); TPmis, 20/22 embryos (91%). For otx2 in late neurulae (2 biological replicates), the pattern in TPMO embryos appeared in 4/4 embryos, and the pattern in TPmis embryos appeared in 10/10 embryos. Image published in: Liu C et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + smad1 MO + NF39 (morphology) fig.6.b Anatomical Phenotype absent head wholly ventralized embryo Xla Wt + smad1 MO + NF39 (morphology) fig.6.c Anatomical Phenotype abnormal bending of tail Xla Wt + smad1 MO + NF39 (morphology) fig.6.c, d Anatomical Phenotype decreased size of the eye decreased size of the head Xla Wt + smad1 MO + NF11 (in situ hybridization) fig.6.h Expression Phenotype decreased amount not.S expression in upper blastopore lip Xla Wt + smad1 MO + NF11 (in situ hybridization) fig.6.i Expression Phenotype decreased amount otx2.S expression in upper blastopore lip Xla Wt + smad1 MO + NF22 (in situ hybridization) fig.6.j Expression Phenotype decreased amount otx2.S expression in eye primordium decreased amount otx2.S expression in forebrain decreased amount otx2.S expression in whole organism Larger Image Printer Friendly View

Return to previous page