Xenbase Image ID: 147634

Fig. 7. Overexpression of miR-26. (A) Embryos were injected with 2 ng/embryo of either miR-26 or the mutated miR-26 (mtt 26); miRs were injected as duplexed oligoribonucleotides. Embryos were cultured until st. 10.5 and collected for RNA isolation and Q-RT-PCR. Genes tested included chordin (chd), not1 (not), vent1 (vn1), vent2 (vn2), EF1-a (EF1), rps18 (r18), and geminin (gmn). N =3 independent experiments. * Indicates statistical significance (p<0.05). (B) In situ hybridization showing expansion of not1 expression in embryos injected with miR-26. N=4 independent experiments; not1 expression patterns are representative of 11/16 (69%) embryos overexpressing miR-26, and 17/17 embryos injected with mtt 26. Image published in: Liu C et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + miR-26 + NF11 (in situ hybridization) fig.7.c Expression Phenotype increased amount not.S expression in upper blastopore lip Larger Image Printer Friendly View

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