Xenbase Image ID: 147635

Fig. 8. Reduction in function of miR-26. (A) Embryos were injected with a cocktail of PLS and Renilla luciferase plus 2 ng of either control LNA (ctrl LNA) or the miR-26 LNA inhibitor; they were collected at st. 10.25–10.5 and lysed for luciferase assays. N=8 experiments. In (A) and (B), * indicates statistical significance (p<0.05). (B) Embryos were injected with 2 ng of either the miR-26 LNA oligonucleotide (LNA) or the control LNA and cultured until control embryos reached st. 10.5; they were then processed for RNA isolation and Q-RT-PCR. Genes tested included vent2, gata4, chordin (chd), goosecoid (gsc), and nodal-related 3 (xnr3). N =3 independent experiments. (C–F) Embryos were injected with oligonucleotides (C,D: 20 ng of either the TPMIS or the TPMO), (E,F: 2 ng of either the control LNA or the miR-26 LNA); ectoderm was isolated from the injected embryos at st.10.25, and ectodermal explants were fixed at st. 11.5 and hybridized in situ to show expression of sox2. Arrowheads indicate explants in which sox2 expression is not detected. (C) explants of ectoderm containing TPMIS. (D) ectoderm injected with TPMO. (E) ectoderm injected with the control LNA; (F) ectoderm injected with miR-26 LNA. Explants are a representative sample of the total; each is oriented to show the maximum level of sox2 expression. (8G) Quantitative comparison of sox2 expression in explants. Numbers in parentheses above the bar indicate the total number of explants. ⁎ Indicates statistical significance (p<0.05; Fisher's Exact Test). N=4 independent experiments. Image published in: Liu C et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

Return to previous page