Xenbase Image ID: 147669

Figure 7. Ectodermin Controls Cell Proliferation via Smad4 (A–E) Immunohistochemical nuclear expression of Ectodermin in normal (A–C) and neoplastic (D, adenomas; E, carcinomas) human colon. (F and G) Effects of Ectodermin depletion on cell proliferation as measured by BrdU incorporation. Green is anti-BrdU; blue is Hoechst. (G) Smad4 is epistatic to Ectodermin. Columns show the effect of Ecto-siRNA depletion on cell proliferation (black bars) relative to control-siRNA-treated cultures (gray bars). The immunoblot lanes on the top confirm the quantitative knockdown of Ecto in each cell line. Only cells wild-type for Smad4, but not Smad4-null cells, are growth arrested upon Ecto downregulation. (H) Depletion of Ecto partially rescues the TGF-β1 (5 ng/ml) growth-suppressing activity in C32 cells. Inset: anti-phospho-Smad2 immunoblot shows that TGF-β1 signaling is effective in C32 cells. Image published in: Dupont S et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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