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Supplemental Figure S6 A lower dose of Ecto-MO (causing a less efficient knockdown, data not shown) enables mesoderm induction by doses of Smad4 mRNA that are otherwise inactive. Two-cell stage embryos were injected with the indicated increasing doses of Smad4 mRNA alone or together with 25 ng of control-MO (-) or Ecto-MO (+). Animal caps were explanted at blastula and harvested for RT-PCR analysis .

Image published in: Dupont S et al. (2005)

Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V.

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