Xenbase Image ID: 147692

Fig. 3. Histological comparison of ptbp1 and exosc9 morphant embryos. Embryos were injected with MoPtbp1 or MoExosc9 in both blastomeres, fixed at stage 40 and processed for histological analysis. (A) Macroscopically, ptbp1 and exosc9 morphant embryos displayed similar blister structures on the dorsal side. (B–Q) 5 µm transversal sections from the embryos presented in A (section plans shown by white line) and stained with hematoxylin/eosin. Detailed images of the dorsal fin (C, G, K, O), and the lateral (D, H, L, P) and ventral (E, I, M, Q) epidermis were shown for each embryo as indicated. Abbreviations: epidermis (ep), neural tube (nt), notochord (nc), somite (sm), sensorial layer cells (slc), ciliated cells (cc) and pigment granule (pg). The scale bar is shown for each image (100 µm, except B, F, J, N 1 mm). Arrowhead points to gap between slc and periderm layer. R) Cells harboring cilia were counted in the dorsal epithelium and normalized to the number of nuclei observed. The number of embryos and sections counted is presented below each barplot. Image published in: Noiret M et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + exosc9 MO + NF40 (morphology) fig.3.a Anatomical Phenotype obsolete abnormally edematous dorsal fin Xla Wt + ptbp1 MO + NF40 (morphology) fig.3.a Anatomical Phenotype obsolete abnormally edematous dorsal fin Xla Wt + ptbp1 MO + NF40 (morphology) fig.3.f Anatomical Phenotype obsolete abnormally edematous dorsal fin Xla Wt + ptbp1 MO + NF40 (morphology) fig.3.g Anatomical Phenotype absent epidermis inner layer Xla Wt + exosc9 MO + NF40 (morphology) fig.3.j Anatomical Phenotype abnormal sensorial layer morphology Xla Wt + exosc9 MO + NF40 (morphology) fig.3.j, n Anatomical Phenotype obsolete abnormally edematous dorsal fin Xla Wt + exosc9 MO + NF40 (morphology) fig.3.k, o Anatomical Phenotype absent epidermis inner layer Xla Wt + exosc9 MO + NF40 (morphology) fig.3.l, m Anatomical Phenotype abnormal sensorial layer morphology Xla Wt + exosc9 MO + NF40 (morphology) fig.3.p, q Anatomical Phenotype absent epidermis inner layer Larger Image Printer Friendly View

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