Xenbase Image ID: 147693

Fig. 4. Scanning Electron Microscopy (SEM) analysis of the peridermal layer of ptbp1 and exosc9 morphant embryos. (A–L) Stage 26 (A, B, E, F, I, J) and stage 36 (C, D, G, H, K, L) embryos, previously injected with MoPtbp1 or MoExosc9 or left uninjected (control) as indicated, were fixed, dehydrated and processed for SEM. The four cell types described in the Xenopus mucociliary epithelium are indicated as ionocytes (i), goblet cells (gc), ciliated cells (cc) and small secretory cells (ssc). (M) The apical area of the goblet cells was measured in stage 36 embryos (MoCo 262 cells, 4 embryos; MoPtbp1 131 cells, 3 embryos; MoExosc9 168 cells, 4 embryos). The scale bar shown on pictures represents 10 µm (A, C, E, G, I, K) or 100 µm (B, D, F, H, J, L). Abbreviations: dorsal fin (df), eye (e), blister (bl). Image published in: Noiret M et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + exosc9 MO + NF35/36 (scanning electron microscopy) fig.4, k, l Anatomical Phenotype increased size of the goblet cell Xla Wt + ptbp1 MO + NF40 (morphology) fig.4.e, f Anatomical Phenotype abnormal goblet cell morphology abnormally increased number of goblet cell Larger Image Printer Friendly View

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