XB-IMG-147713
Xenbase Image ID: 147713
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Figure 1. Genetic relationship between human and Xenopus Klf factors and temporal expression analysis of klf genes. A: Phylogenetic analysis using protein sequences of human KLF1 (Protein ID: Q13351), KLF2 (Protein ID: Q9Y5W3), KLF3 (Protein ID: P57682), KLF4 (Protein ID: O43474), KLF5 (Protein ID: Q13887), KLF6 (Protein ID: Q99612), KLF7 (Protein ID: O75840), KLF8 (Protein ID: O95600), KLF9 (Protein ID: Q13886), KLF10 (Protein ID: Q13118), KLF11 (Protein ID: O14901), KLF12 (Protein ID: Q9Y4X4), KLF13 (Protein ID: Q9Y2Y9), KLF14 (Protein ID: Q8TD94), KLF15 (Protein ID: Q9UIH9), KLF16 (Protein ID: Q9BXK1), KLF17 (Protein ID: Q5JT82), Xenopus laevis Klf2 (Genbank Accession number: NP_001080430), Xenopus tropicalis Klf3 (XP_002938694), Xenopus laevis Klf4 (AEK12773), Klf5 (Encoded by Gurdon EST transcript: Xl3.1-IMAGE: 6955854.5), Klf6a (Encoded by cDNA with Accession number: KP013621), Klf7 (NP_001080906), Klf8 (NP_001082615), Klf9 (NP_001079066), Klf10 (NP_001089340), Klf11 (NP_001086010.1), Xenopus tropicalis Klf12 (Ensembl: ENSXETP00000010232), Klf13 (NP_001093690.1), Xenopus laevis Klf15 (NP_001084413.1), and Klf17 (NP_001082133). Phylogenetic tree was generated using the program Clustal Omega at www.ebi.ac.uk. B: Comparison of colinearity of genes flanking KLF17 in human and klf17 in Xenopus tropicalis. Diagram was drawn according to the gene colinearity from the Ensembl genome browser (http://asia.ensembl.org). C: Temporal expression patterns of the klf genes during the embryogenesis of Xenopus laevis as detected with semi-quantitative RT-PCR. odc (ornithine decarboxylase) was used as loading control. gsc (goosecoid) was used as a control to show the absence of maternal transcription. RT-, reverse transcription without reverse transcriptase.Download figure to PowerPoint Image published in: Gao Y et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |