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Figure 4. Effects of overexpression of Klf factors on embryonic development and germ layer formation. A–K: Injection of each 500 pg mRNA for Klf factors, as indicated in each panel, generated different effects on embryonic development. Lateral view is shown for uninjected control (Uninj.) and injected embryos. (Putative) dorsal is up and anterior is to the left. L: Ratios (percentages) of normal and defect embryos among the total numbers (n) of control (ctrl) embryos or embryos injected with Klf mRNAs. M: Analysis on the expression of t, sox17a and sox2 in control (ctrl) embryos and embryos injected with different Klf mRNA. Lateral view is shown for embryos with t expression, and animal pole is up. Both animal (a. view) and vegetal view (v. view) are shown for the embryos with sox17a expression. Dorsal view is shown for embryos with sox2 expression. N: Ratios (percentages) of the embryos with regular gene expression or misregulated gene expression among the total numbers (n) of control (ctrl) or injected embryos.Download figure to PowerPoint Image published in: Gao Y et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, John Wiley & Sons.
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