Xenbase Image ID: 147718

Figure 6. Effects of knockdown of Klf factors on embryonic development. A: Western blotting analysis on the efficiency of control MO (ctrlMO) and Klf MOs to inhibit protein translation in Xenopus embryos. Blots for Actin were similar for all the samples and only one of them was shown as control. A total of 500 pg of each mRNA and each 40 ng of ctrlMO, K2MO, K5MO, K6MO, K7MO, K15MO, 15 ng of K8MO, and 50 ng of K11MO were injected. B,H: Embryos injected with ctrlMO at tail bud (B) and tadpole (H) stages, respectively. C–G,I–J: Developmental defects after injection of different Klf MOs, as indicated in each panel. Lateral view is shown for all embryos, with the anterior being orientated to the left. ctrlMO, K8MO, K11MO, and K15MO were injected at 50 ng each, K2MO was injected at 30 ng, K5MO was 80 ng, K6MO was 40 ng, and K7MO was injected at 15 ng. K: Percent of normal or defect embryos among the total numbers (n) of each morpholino injected embryos.Download figure to PowerPoint Image published in: Gao Y et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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