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Figure 9. Effect of double knockdown on gene expression. A: Effect of single knockdown and double knockdown of Klf2 and Klf7 on the expression of sox17a. B: Effect of single knockdown and double knockdown of Klf7 and Klf8 on the expression of gsc. C: Ratios of embryos with normal or disturbed transcription among the total numbers (n) of injected embryos. ctrlMO, K2MO, and K7MO were injected at 30 ng per embryo, and K8MO was injected at 50 ng per embryo. l, lateral view with animal pole to the top of each panel; v, vegetal view with dorsal side to the top of each panel.Download figure to PowerPoint Image published in: Gao Y et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, John Wiley & Sons.
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