Xenbase Image ID: 147723

FIGURE 1. Expression of ets1 in NC is regulated by Lrig3 and FGF signaling. A, embryos were injected with chordin (Chd), chordin + wnt3a, or chordin + wnt3a + lrig3MO (L3MO). Animal caps were dissected at stage 9 and cultured to stage 17. Expression of the indicated genes in animal caps was assayed by RT-PCR. Ornithine decarboxylase (odc) was used as an internal standard. WE, uninjected whole embryo; AC, uninjected animal caps; RT−, without reverse transcriptase. B–G, spatial expression pattern of ets1 as detected by in situ hybridization. B, C, and D, dorsal view; D′, anterior view; E, F, and G, lateral view. The white arrow in C indicates the weak ets1 signal starting at stage 13. The ventral blood island is indicated by a white arrow in D′. H, temporal expression of Xenopus ets1a at the indicated stages. I–N, embryos treated with the indicated concentrations of SU5402 from stage 12. ets1 and brachyury (bra) expression was examined at stage 16 by whole-mount in situ hybridization. The expression of the indicated genes was affected in the following percentage of embryos: M, 73% (8 of 11); N, 100% (20 of 20); J, 33% (7 of 21); K, 61% (11 of 18). O, embryos were injected with 10 pg of efgf or 300 pg of wnt3a mRNA, respectively. The caps were dissected from embryos at stage 9 and then cultured to stage 15. Expression of the indicated genes in animal caps was assayed by RT-PCR. Image published in: Wang C et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue ets1.S c-ets1b, ets-1, ewsr2, xe1-a X. laevis Throughout NF stage 16 cranial neural crest ets1.S c-ets1b, ets-1, ewsr2, xe1-a X. laevis Throughout NF stage 13 neural crest cranial neural crest premigratory neural crest cell ets1.S c-ets1b, ets-1, ewsr2, xe1-a X. laevis Throughout NF stage 16 neural crest cranial neural crest premigratory neural crest cell ventral mesoderm ets1.S c-ets1b, ets-1, ewsr2, xe1-a X. laevis Throughout NF stage 22 neural crest cranial neural crest postmigratory neural crest cell hyoid crest branchial crest anterior branchial crest posterior branchial crest ets1.S c-ets1b, ets-1, ewsr2, xe1-a X. laevis Throughout NF stage 28 neural crest cranial neural crest postmigratory neural crest cell head mesenchyme lateral plate mesoderm ventral blood island ets1.S c-ets1b, ets-1, ewsr2, xe1-a X. laevis Throughout NF stage 37 and 38 neural crest cranial neural crest cardiac neural crest lateral plate mesoderm dorsal lateral plate mesoderm head mesenchyme ets1.S c-ets1b, ets-1, ewsr2, xe1-a X. laevis Throughout NF stage 12 neural crest tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 12 circumblastoporal collar involuted dorsal mesoderm involuted ventral mesoderm Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + su5402 + NF16 (in situ hybridization) fig.1.j Expression Phenotype decreased amount ets1.L expression in neural crest Xla Wt + su5402 + NF16 (in situ hybridization) fig.1.k Expression Phenotype decreased amount ets1.L expression in neural crest Xla Wt + su5402 + NF16 (in situ hybridization) fig.1.m Expression Phenotype decreased amount tbxt.S expression in anterior neural fold Xla Wt + su5402 + NF16 (in situ hybridization) fig.1.n Expression Phenotype decreased amount tbxt.S expression in anterior neural fold Larger Image Printer Friendly View

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