Xenbase Image ID: 147728

FIGURE 6. Ets1 physically interacts with HDAC1 through the ETS domain. A, co-IP was carried out with extracts of HEK293T cells transfected with either Ets1-Myc, HDAC1-HA separately, or both using antibodies against Myc or HA. B, co-IP indicates that Ets1-Myc binds to HDAC1-HA in Xenopus embryos. C–E, expression patterns of Xenopus hdac1 (C) and ets1 (D) in Xenopus embryo at stage 17. A schematic diagram of Xenopus hdac1 and ets1 expression (E) indicates overlap between them. F, Ets1 deletion mutants and their binding ability to HDAC1. G, co-IP was carried out to examine the binding of Myc-tagged Ets1 deletion mutants and HDAC1-HA in HEK293T cells. IB, immunoblot; TAD, transactivation domain; SAM, sterile α motif; PNT, pointed. Image published in: Wang C et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue hdac1.L gon-10, hdac1-a, hdac1-b, rpd3, rpd3l1 X. laevis Throughout NF stage 17 neural plate pre-chordal neural plate chordal neural plate neural plate border ets1.S c-ets1b, ets-1, ewsr2, xe1-a X. laevis Throughout NF stage 17 neural crest cranial neural crest premigratory neural crest cell Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + ets1del_783-1314 + NF17 (in situ hybridization) fig.7.b Expression Phenotype mislocalised foxd3.L expression in neural plate border increased amount foxd3.L expression in neural plate border Xla Wt + ets1del_1044-1314 + NF17 (in situ hybridization) fig.7.c Expression Phenotype mislocalised foxd3.L expression in neural plate border increased amount foxd3.L expression in neural plate border Xla Wt + ets1del_1-450 + NF17 (in situ hybridization) fig.7.d Expression Phenotype decreased amount foxd3.L expression in neural crest Xla Wt + ets1del_1-906 + NF17 (in situ hybridization) fig.7.e Expression Phenotype decreased amount foxd3.L expression in neural crest Xla Wt + ets1del_1-450 + NF17 (in situ hybridization) fig.7.i Expression Phenotype decreased amount krt12.4.L expression in non-neural ectoderm Xla Wt + ets1del_1-906 + NF17 (in situ hybridization) fig.7.j Expression Phenotype decreased amount krt12.4.L expression in non-neural ectoderm Larger Image Printer Friendly View

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