Xenbase Image ID: 147732

Fig. 1. Isolation and expression of Xenopus PN1. (A) SDS-PAGE of supernatant from transfected and [35S]methionine/cysteine-labeled HEK293T cells. The 45 kDa band corresponds to secreted PN1.a protein. (B) RT-PCR of PN1.a, PN1.b and HtrA1 in Xenopus embryos. Histone H4 was used for normalization. (C-K′) Whole-mount in situ hybridization of PN1.a, PN1.b and HtrA1 at stage 10 (C-E, hemisections), stage 13 (F-H, dorsal views; F′-H′, hemisections) and stage 26 (I-K, lateral views; I′-K′, transverse sections). Dashed lines indicate section planes. anp, anterior neural plate; ea, ear; ec, ectoderm; m, mesoderm; nc, neural crest; no, notochord; pm, posterior mesoderm; sc, spinal cord. (L-N) Lateral view of stage 10 embryos. A single injection of 9 pg Fgf4 mRNA stimulates (arrow) and 450 pg XFD mRNA downregulates PN1.a expression. nlacZ mRNA was co-injected as lineage tracer (red nuclei). The frequency of embryos with the indicated phenotypes was: L, 67/67; M, 57/63; N, 47/53. (O) RT-PCR of PN1.a and PN1.b in animal cap (AC) explants injected with 12 pg Fgf4 and 4.5 ng XFD mRNA (n=3 replicates). Image published in: Acosta H et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue serpine2.L gdn, glia-derived nexin, pn-1, pn1, Protease nexin-1, xpn-1 X. laevis Throughout NF stage 10 neuroectoderm mesoderm ectoderm serpine2.L gdn, glia-derived nexin, pn-1, pn1, Protease nexin-1, xpn-1 X. laevis Throughout NF stage 13 pre-chordal neural plate serpine2.L gdn, glia-derived nexin, pn-1, pn1, Protease nexin-1, xpn-1 X. laevis Throughout NF stage 26 notochord axial mesoderm htra1.S high temperature required A1, prss11, Xhtra1, xHtrA1 X. laevis Throughout NF stage 10 ectoderm neuroectoderm mesoderm htra1.S high temperature required A1, prss11, Xhtra1, xHtrA1 X. laevis Throughout NF stage 13 neural plate pre-chordal neural plate chordal neural plate mesoderm lateral plate mesoderm axial mesoderm htra1.S high temperature required A1, prss11, Xhtra1, xHtrA1 X. laevis Throughout NF stage 26 notochord paraxial mesoderm neural crest Image source: Published Larger Image Printer Friendly View

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