XB-IMG-147742
Xenbase Image ID: 147742
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Figure S3. Overexpression of PN1 and Dkk1 promote anterior development
(A) Uninjected embryo at tailbud stage.
(B-D) PN1.a, PN1.b, and Dkk1 mRNA induce enlarged head and shortened trunk/tail
structures.
(E,F) PN1.b and Dkk1 mRNA widen eye structures in tadpole embryos. Insets show
magnification of eye. Note the expansion of the optic fissure (arrowhead) that is characteristic
of coloboma.
Embryos were animally injected at the 4-cell stage with mRNAs: PN1.a, 4 ng; PN1.b, 16 ng;
Dkk1, 32 pg. Indicated phenotypes were observed in B, 40/54; C, 23/23; D, 34/34; E, 26/31;
F, 46/53. Image published in: Acosta H et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher. Larger Image Printer Friendly View |