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Figure S5. Knockdown of PN1 delays gastrulation Embryos were animally injected with 60 ng MOs at the 4-cell stage. Embryos are shown in vegetal view, dorsal to the top, from the onset (left) to the end of gastrulation (right). Vertically aligned embryos are time-matched. (A-E) Control-MO-injected embryos undergo normal gastrulation, which results in full closure of the circular blastopore. (F-J) PN1-MO does not affect the onset of gastrulation at stage 10 (F), but causes a delay in blastopore closure at subsequent stages. Pictures are representative of a minimum of 20 analysed embryos. Image published in: Acosta H et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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