XB-IMG-147746
Xenbase Image ID: 147746
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Figure S7. PN1 promotes anterior development via inhibition of HtrA1-, FGF- and Wnt
signals
Embryos were animally injected with MOs at the 2-cell stage and mRNAs at the 4-cell stage.
(A) Anterior view of early neurula embryos after whole-mount in situ hybridization with
Otx2, demarcating the prospective cement gland, forebrain and midbrain.
(B) PN1 mRNA (4 ng) causes expansion of Otx2 expression.
(C,D) PN1-MO (60 ng) reduces the Otx2 marker, while an equivalent amount of control-MO
has no effect.
(E,F) HtrA1-MO (20 ng) or 80 pg δXFGFR-4a mRNA restore normal Otx2 expression in
PN1 morphant embryos.
Indicated phenotypes were observed in B, 12/13; C, 59/62; D, 63/73; E, 22/24; F, 23/29. Image published in: Acosta H et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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