Xenbase Image ID: 147746

Figure S7. PN1 promotes anterior development via inhibition of HtrA1-, FGF- and Wnt signals Embryos were animally injected with MOs at the 2-cell stage and mRNAs at the 4-cell stage. (A) Anterior view of early neurula embryos after whole-mount in situ hybridization with Otx2, demarcating the prospective cement gland, forebrain and midbrain. (B) PN1 mRNA (4 ng) causes expansion of Otx2 expression. (C,D) PN1-MO (60 ng) reduces the Otx2 marker, while an equivalent amount of control-MO has no effect. (E,F) HtrA1-MO (20 ng) or 80 pg δXFGFR-4a mRNA restore normal Otx2 expression in PN1 morphant embryos. Indicated phenotypes were observed in B, 12/13; C, 59/62; D, 63/73; E, 22/24; F, 23/29. Image published in: Acosta H et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher. Gene Synonyms Species Stage(s) Tissue otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 14 optic field pre-chordal neural plate Image source: Published Larger Image Printer Friendly View

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