XB-IMG-147747
Xenbase Image ID: 147747
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Figure S8. Knockdown of PN1 promotes mesoderm and suppresses ectoderm formation
in an HtrA1- and FGF-dependent manner
Embryos were separately injected with MOs and mRNAs into the animal pole at the 4-cell
stage. Hemisectioned early gastrula embryos are shown after whole-mount in situ
hybridization for Xbra (A-F) and Foxi1e (A’-F’).
(A-B’) PN1-MO, but not control-MO, induces ectopic Xbra and reduction of Foxi1e
expression in deep cells of the animal hemisphere (brackets in insets depict animal cap).
(C-D’) Injections of 200 pg HtrA1 and 2 pg Fgf4 mRNA induce ectopic Xbra at the expense
of Foxi1e expression in the inner layer of the animal cap (insets).
(E-F’) Injections of HtrA1-MO and 80 pg XFD mRNA restore normal expression of Xbra and
Foxi1e in PN1-morphant embryos.
Total injected MO amount was 60 ng (+20 ng HtrA1-MO). The frequency of the indicated
phenotypes was A, 136/144; A’, 50/59; B, 148/177; B’, 50/69; C, 21/21; C’, 47/57; D, 79/79;
D’, 93/97; E, 66/90; E’, 52/56; F, 72/83; F’, 45/51. Image published in: Acosta H et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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