XB-IMG-147758
Xenbase Image ID: 147758
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Fig. 7. Quantitation of FN fibril elongation. (a) Maximum fibril length as a function of elongation time. Double-staining experiments (DS) were
performed as described (Fig. 4a). The average length of the three largest newly formed fibrils for each time point is indicated, and the range
between the longest and the shortest of these fibrils is represented as a vertical bar. In a second approach, stage 9 blastocoel roofs were used to
condition substratum for varying times (CS). For each time point, the 3 longest fibrils were selected, and treated as above. The increase in
maximum fibril length during the first 4 minutes (DS) or 3 minutes (CS), respectively, is 4.7 mm/min in both cases. (b) Frequency distribution
of fibril lengths. The lengths of newly added segments of elongating fibrils as seen in double-labelling experiments were measured as described
above. Fibril elongation was allowed for 2 minutes (top; 57 cases of fibril elongation evaluated) or for 8 minutes, respectively (bottom; 78
cases), and fibril lengths were classed according to 2 mm steps. Image published in: Winklbauer R and Stoltz C (1995) Copyright © 1995. Image reproduced with permission of the Publisher, The Company of Biologists Ltd. Larger Image Printer Friendly View |