Xenbase Image ID: 147763

Fig. 2. (a) FGF-8 induces ectopic N-tubulin without inducing ectopic collagen type II. Control and FGF-8 RNA-injected embryos were hybridised with probes to collagen type II and N-tubulin (doubly or singly, as indicated) and sectioned. In all embryos, ectopic N-tubulin (black arrow) was not underlain by ectopic collagen type II. (b) Qualitatively different effect of eFGF and FGF-8 on Xbra expression. Injection of eFGF/lacZ RNA expanded Xbra whereas injection of FGF-8/lacZ RNA suppressed Xbra. Control lacZ injections had no effect on Xbra (inset). (c) Suppression of Xbra by FGF-8 and induction of Xbra by eFGF is dose dependent. A representative experiment is shown. The concentration difference between the low and high dose was threefold for both FGF-8 and eFGF. Expansion of Xbra in response to FGF-8 differed from that obtained with eFGF in that it was weaker in intensity and did not extend to the animal pole. In some FGF-8 RNA-injected embryos, both suppression and expansion of Xbra was observed in the same embryo. Low FGF-8, n=14; high FGF-8, n=15; low eFGF, n=16; high eFGF, n=24. Image published in: Hardcastle Z et al. (2000) Copyright © 2000. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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