Xenbase Image ID: 147783

Figure 1. Karyotyping Xenopus tropicalis and Xenopus laevis tadpoles and the effects of vector sequences on transgene expression. A: A typical diploid karyotype from an X. tropicalis tadpole. Note the small, submetacentric pair of chromosomes (arrows) that is characteristic of X. tropicalis. B: A typical karyotype from X. laevis, provided for comparison. C: Stage 32 embryos transgenic for a Rx/green fluorescent protein (GFP) construct including the pEGFP-1 plasmid vector sequence. Normal expression is detected in the eyes (arrow), but ectopic expression in the axial muscle (arrowheads) is quite strong. D: Stage 32 embryos transgenic for Rx/GFP using the Rx promoter/GFP/polyA cassette only. Note that normal Rx expression is unaffected (arrows), but the ectopic expression is eliminated. Image published in: Hirsch N et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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