Xenbase Image ID: 147786

Figure 4. Late-stage tadpoles reveal complex patterns of transgene expression. Dorsal view, anterior to the left in all panels. A:Pax-6/green fluorescent protein (GFP) transgenic tadpole at stage 47. GFP expression is strong in the brain and in other nervous tissue. The arrowhead with asterisk shows the olfactory placodes, which are strongly GFP positive. Arrows indicate the olfactory and optic nerves, whereas the arrowheads indicate neurons of the lateral line. B:Otx2/GFP transgenic tadpole at stage 45, showing complex, segmental expression in the hindbrain (arrowhead with asterisk) as well as in the forebrain (arrowhead). The eye still strongly expresses GFP even at this late stage (arrow). C: An EF1α/GFP transgenic tadpole at stage 45. Expression is strong in the axial muscle (posterior arrowhead) and in muscles of the pharynx (anterior arrowhead). Brain expression appears regionalized (arrows), although that is likely an effect of the thickness of different brain tissues. Image published in: Hirsch N et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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