Xenbase Image ID: 147789

The ubiquitously expressed EF1α/green fluorescent protein (GFP) transgene can be used as a host/donor marker in transplant and recombinant experiments. A:EF1α/GFP-expressing transgenic tadpole at stage 32, highlighting the widespread expression. B: A wild-type host embryo with an EF1α/GFP ectodermal transplant ventrally. The boundaries of the transplant are marked by arrowheads. C,D: A transplant of the presumptive lens ectoderm from a stage 14 EF1α/GFP transgenic donor to the lens region of a stage 14 wild-type host. C, brightfield; D, fluorescence. The arrows in C,D indicate the lens induced from donor tissue. All panels are lateral views; anterior is to the right. Image published in: Hirsch N et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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