Xenbase Image ID: 147795

Fig. 1. A pilot microarray identifies genes whose expression changes during early X. tropicalis development. The microarrays were probed with labelled (Cy3 or Cy5) cDNA from st3 (4 cell) and stage 19 (neurula) X. tropicalis embryos. The hybridisations were carried out 4 times with one combination of dyes and 4 times with the dyes swapped, giving eight hybridisations in total. Each hybridisation is a biological replicate. The arrays were then scanned and the log2 ratio of fluorescence calculated for each oligonucleotide. (A) Cluster analysis of eight hybridisations using X. tropicalis material. Each column represents the result from 1 hybridisation, each bar the results from one gene. The greater the intensity of colour the greater the difference in ratio of the two wavelengths. Genes that appear black are not expressed or are evenly expressed in the two stages. Clusters of genes with higher expression at st3, st19 or equal expression can be identified. (B) The mean of the log2 ratio of the two wavelengths is plotted for each gene. The genes show a gradual transition from highly expressed at stage 3 to highly expressed in stage 19. The position of 3 well known Xenopus genes is marked. Image published in: Chalmers AD et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

Return to previous page