Xenbase Image ID: 147886

Fig 6. Knockdown of endogenous Cerberus enhances the head phenotypes induced by microinjection of McerP-BMP4, -Xnr1 and -Xwnt8. (A-D) The head defects observed by the coinjection of McerP-BMP4, -Xnr1 and -Xwnt8 (8 pg each) together with the CoMo (B; 64%, n=62) can be aggravated when endogenous cerberus is knock-down by 1.6 pmol of CerMo (C; 65%, n=46). The specificity of this sensitization was verified by the coinjection of Cer-Long plasmid, which could rescue the phenotype (D; 58%, n=42). (E-J) The mild phenotypes obtained by individually injecting McerP-BMP4 (E; 30%, n=44), McerP-Xnr1 (G; 40%, n=40) or McerP-Xwnt8 (I; 66%, n=47) were also aggravated by the coinjection of 1.6 pmol CerMo (F; 25%, n=41. H; 60%, n=39. J; 58%, n=44 respectively). (K-L) CerMo dependent aggravation of the McerP-Xwnt8 phenotype could be completely rescued by Cer-Long construct (K; 66%, n=48), but only partially rescued if Cer-Short plasmid (L; 55%, n=44) is used instead. These results were observed in two independent experiments. (M) Graphical representation of the range of phenotypes observed by increasing amounts of BMP, Wnt and Nodal misexpressed in the ADE, showing the requirement of lower amounts of these signals to generate the same phenotypes when endogenous Cerberus is depleted. Image published in: Silva AC et al. (2003) Copyright © 2003. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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