Xenbase Image ID: 147892

Characterization of XRnf12. (A) Amino acid sequence alignment of XRnf12, XRnf12B, and XRnf12C, as well as mouse and chick Rnf12. Note the repetitive heptamers present in XRnf12B and XRnf12C. A PDZ domain-binding motif is present in the C terminus. NLS, nuclear localization signal; asterisks, sites of mutation in the RING mutant XRnf12(HC>AA). DDBJ Accession Numbers are: XRnf12 (AB114039), XRnf12B (AB114040) and XRnf12C (AB114041). (B) Schematic representation of mRnf12, XRnf12, XRnf12B, and XRnf12C. (C) The N-terminal region (aa 1-283) of XRnf12 interacts with LIM homeodomain proteins and Ldb1. GST pull-down assay was performed with 35S-labeled Xlim-1, Xlim-3, Xlim-5, Ldb1 and Ldb1ΔC. While GST XRnf12ΔC interacts with all of the proteins tested, GST XRnf12ΔN shows little interaction. GST and GST Ldb1 serves as negative and positive controls, respectively. Image published in: Hiratani I et al. (2003) Copyright © 2003. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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