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Ldb1 overexpression affects the maintenance phase of gsc expression. Embryos injected dorsally with the mRNAs (4.0 ng/embryo) indicated were collected at stages 9.5, 10, 10.5 and 11 (late blastula to mid gastrula) and the expression of gsc was analyzed by RT-PCR. Downregulation of gsc by Ldb1 overexpression was not prominent at early-gastrula stages (st 9.5, 10) but became prominent after mid gastrula (stage 10.5, 11). Co-expression of XRnf12 restored gsc expression. EF-1α serves as a loading control. Image published in: Hiratani I et al. (2003) Copyright © 2003. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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