XB-IMG-147903
Xenbase Image ID: 147903
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Figure 2.
Structure-Function Relationship of XPK: Overexpression of Wild-Type and XPK Deletion Constructs
(A) Structures of wild-type XPK and deletion constructs; ΔPET, ΔLIM, ΔP/L, and P/L are illustrated in (a). (b) All of these constructs inhibit activin-induced elongation of animal cap explants (wild-type and ΔPET, 500 pg; ΔLIM, 1 ng; ΔP/L and P/L, 250 pg). (c) None of the XPK constructs affect activin-induced Xbra and Xwnt11 expression in animal cap explants at stage 10.5. The indicated numbers correspond to (b1–6).
(B) All of the constructs perturb normal gastrulation movements when injected into the dorsal embryo. Phenotypes of abnormal gastrulation were classified into three grades that indicated (a) (1) a normal or weak phenotype showing a small head but nearly normal-sized trunk and tail, (2) an intermediate phenotype showing a short and curved body axis, or (3) a severe, spina bifida-like phenotype showing an open blastopore. (b) Effects of LIM domain-deleted Xpk injection are rescued by the coinjection of wild-type Xpk mRNA. In this bar graph, the phenotypes caused by injection of these constructs into the dorsal embryo were scored as 1, 2, or 3 and are indicated with white, gray, and black color, respectively. (c) An example of phenotype rescue. The result of injecting ΔP/L at 250 pg is shown in the upper panel, and the phenotype resulting from injecting ΔP/L with wild-type Xpk at 500 pg is shown in the lower panel. The ratio of abnormal embryos was reduced by the coexpression of wild-type Xpk. Image published in: Takeuchi M et al. (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |