Xenbase Image ID: 147904

Figure 3. XPK Interacts Physically with Dishevelled and JNK to Activate JNK in HEK293T Cells (A) JNK activation could not be detected in Xpk-transfected cells, but Xdsh alone could activate JNK in a dose-dependent manner. (B and C) Two-phase XPK activity, depending on the Xdsh dose. (B) At a low Xdsh dose, wild-type XPK enhances Xdsh-mediated JNK activation. (C) At a high Xdsh dose, wild-type XPK or ΔP/L suppresses the activation of JNK, which is mediated by Xdsh. HEK293T cells were transfected with the indicated expression plasmids. The activation of JNK was monitored with an anti-phosphorylated c-Jun antibody. Western blotting with an anti-Gal4DBD antibody was the control for the transfection and loading quantity. (D) XPK interacts physically with Xdsh. (E) Wild-type XPK interacts physically with JNK1. Image published in: Takeuchi M et al. (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

Return to previous page