Xenbase Image ID: 147924

Fig. 1. The Xfz7 transmembrane receptor is expressed in Xenopus embryos. (A) Schematic representation of the predicted Xfz7 structure. The protein contains a putative N-terminal signal peptide, a cysteine-rich domain (CRD) containing 10 cysteine residues, seven transmembrane domains with seven hydrophobic stretches, and a small C-terminal tail of 26 amino acids with a S/T-X-V motif region. Recently, Xfz7 cDNA sequences have been reported in GenBank database by other groups (accession numbers: AJ243323, AF114151 and Wheeler and Hoppler, 1999). (B) Temporal expression of Xfz7 mRNA during Xenopus development. Northern blot analysis was carried out using total RNA from mature oocytes (stage VI) and different embryonic stages (1.5 oocyte/embryo equivalent). The full length Xfz7 cDNA labelled with 32P was used as hybridization probe. A 4-kb Xfz7 transcript is expressed in oocytes and persists during early cleavage stages. Zygotic transcripts peak at stage 11. As loading control, the 18S Ribosomal RNA was stained with ethidium bromide. Image published in: Medina A et al. (2000) Copyright © 2000. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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