Xenbase Image ID: 147928

Fig. 5. Overexpression of Xfz7 affects morphogenetic movements in animal caps and dorsal lip explants. (A) Experimental scheme. Embryos were coinjected at the four-cell stage with 0.5 ng of Xfz7 and 0.1 ng of β-gal mRNA as a lineage tracer. Animal caps were dissected at stage 8.5 and in the presence of activin protein and analyzed for elongation at stage 17. (B) Animal cap control explants from uninjected embryos treated with activin (elongation in 95%, n=18). The small panel shows uninduced explants. (C) In animal cap explants overexpressing Xfz7 and β-gal and stimulated with activin, elongation is inhibited (82%, n=62). (D) Pigmented embryos were microinjected at the four-cell stage in the left dorsal marginal zone with 0.25 ng of dextran fluorescein fluoro-emerald (DG) and 0.5 ng of Xfz7 mRNA with 0.5 ng of dextran blue (DB) was injected in the right dorsal marginal zone. The embryos were cultured until stage 10.5 and dorsal explants were microdissected and cultured for 5–7 h. (E) Stage 10.5, vegetal view. Distribution of the fluorescent markers (DG and DB) in the blastopore lip before explantation. (F) Open-faced dorsal explant from a control embryo injected on the right side with DB and on the left side with DG. (G) Open-faced dorsal explant from embryo injected on the right side with Xfz7 mRNA plus DB and DG on the left side. Elongation was inhibited on the side that received the Xfz7 mRNA. Image published in: Medina A et al. (2000) Copyright © 2000. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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