XB-IMG-147930
Xenbase Image ID: 147930
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Fig. 7. Interaction of Xfz7 with Wnt-1 and Wnt-5a-type ligands. (A) Increasing amounts of Xfz7 mRNA (0.5–2 ng) were injected into animal blastomeres. Animal caps were explanted at stage 8.5 and cultured until stage 10. Expression of the dorsal marker siamois was assayed by RT–PCR. (B) Synthetic Xwnt-5a type-ligands (0.02 ng) (Xwnt-4, Xwnt-5 and Xwnt-11 mRNAs) alone or in combination with 0.5 ng of Xfz7 mRNA were injected into two animal blastomeres. Animal caps were explanted at stage 8.5 and cultured until stage 10 and expression of sia and Xnr-3 was analyzed by RT–PCR. Xwnt-8 mRNA (0.02 ng) alone but not Xwnt-5a-type ligands in combination with Xfz7 induced Xnr-3 and sia expression. (C) Xwnt-8b acts synergistically with Xfz7 to trigger the Wnt-1 pathway. Synthetic Xwnt-8 mRNA (0.02 ng), Xwnt-8b mRNA (0.02–0.1 ng) alone or in combination with 0.5 ng of Xfz7 or 0.5 ng of δCXfz7 mRNAs were microinjected into two animal blastomeres at the four-cell stage. Animal explants were dissected and analyzed for Xnr-3 expression by RT–PCR. Image published in: Medina A et al. (2000) Copyright © 2000. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |