Xenbase Image ID: 147931

Fig. 8. Overexpression of Xfz7 triggers the localization of GFP-PKC to the membrane. (A) Embryos were injected into the animal pole with 0.4 ng GFP-PKC mRNA together with 0.05 ng of Xwnt-5a or (B) 0.25 ng Xfz7 or (C) 0.25 ng NXfz7-fun mRNA. At stage 10 the animal caps were explanted, fixed and the localization of the GFP fluorescence was determined. Membrane staining was observed in 77% (n=26) of the caps injected with Xwnt-5a and in 68% (n=38) of the caps expressing Xfz7 but only 11% (n=18) of the explants expressing NXfz7-fun displayed weak PKC membrane staining. Image published in: Medina A et al. (2000) Copyright © 2000. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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